Feed Mitigant Efficacy for Control of Porcine Epidemic Diarrhea Virus and Porcine Reproductive and Respiratory Syndrome Virus when Inoculated Alone or Together in Feed

Research has demonstrated that swine feed can be a fomite for viral transmission and additives reduce contamination. Therefore, the objective of this study was to evaluate two in contaminated with PEDV or PRRSV. Feed included: no treatment, 0.33% commercial formaldehyde-based product, 0.50% medium chain fatty acids (MCFA) blend. samples were inoculated PRRSV alone together at an inoculation concentration 106 TCID50/g each virus. Once inoculated, stored room temperature 24 h before analyzing via qRT-PCR. For alone, quantitative real time reverse transcription PCR (qRT-PCR) assay used, which designed detect nucleic acid. co-inoculated samples, within single assay. there marginally significant evidence resulted differences cycle threshold (Ct) value (P = 0.052), but observed pairwise differences. formaldehyde increased Ct compared untreated control MCFA treatment < 0.05). co-infection PEDV, 0.05) comparison non-treated feed. In summary, values when while both PEDV. This also provided co-inoculation model effectively mitigants.